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UA-Zero® EM Stain is a patented solution developed as a direct substitute for Uranyl Acetate. UA-Zero does not contain any radioactive material and is non-toxic. It can be used as a direct replacement for Uranyl Acetate with no changes to standard user protocols and delivers high contrast imaging.

· Uranyl Acetate substitute

· Non-radioactive, Uranium free

· No handling, storage or disposal restrictions

· Image contrast results as good as Uranyl Acetate, if not better

· No additional processing required

· Direct replacement for Uranyl Acetate without changing standard user protocols

· Independently tested and verified

UA-Zero is non-radioactive and Uranium free. Consequently, there are none of the handling, storage or disposal issues associated with Uranyl Acetate.

The ready-to-use lead-free solution can be used as a direct replacement for to Uranyl Acetate without any changes to the standard user protocols, and no additional processing is required to enhance the image contrast. As shown in the Application Notes the image contrast results carried out at Bristol University are as good as Uranyl Acetate, if not better.

UA-Zero is supplied in an opaque dropper bottle, which should be stored long-term in the lab fridge at around 4°C. For daily use, the sealed bottle can be stored in normal laboratory conditions away from direct sunlight.

It has been shown that UA-Zero EM Stain works very well for various protocols that specify Uranyl Acetate as a staining solution for TEM sample preparation in life science. The specific protocols described in the Application Notes are examples for preparing samples and subsequent imaging using TEM. Uranyl Acetate and UA-Zero EM stain were directly compared and the images generated are shown in the Results sections.

UA-Zero is easy to use; to stain the sample on a TEM grid, with a support film, only a few simple steps are required before drying.

UA-Zero is supplied in 25ml bottles.

Technical Data

In collaboration with Bristol University we have produced several Application Notes detailing the protocols and processes used to prepare stained specimens using both UA-Zero and Uranyl Acetate. Each note contains TEM images comparing the results from UA-Zero with Uranyl Acetate.

	Fixation, staining & processing of HeLa cell pellet from culture dish
	Fixation, staining & processing of HeLa cells grown on coverslips
	Fixation, staining & processing of kidney tissue
	Tobacco Mosaic Virus (TMV) Negative staining

MSDS

UA-Zero is labelled with the appropriate hazard symbols and risk & safety phrases in accordance with current UK regulations and EC directives.

A Material Safety Data Sheets (MSDS)/Safety Data Sheets (SDS) is available to assist in the safe handling and storage of the product and the completion of any requirements under COSHH.

 AGR1000 UA-Zero EM Stain MSDS